The n Counter Analysis System utilizes a novel digital color-coded barcode technology that is based on direct multiplexed measurement of gene expression and offers high levels of precision and sensitivity ( Each color-coded barcode represents a single target molecule.
Barcodes hybridize directly to your target molecules and can be individually counted without the need for amplification—providing sensitive digital data.
The aim of this study is to identify and characterize lnc RNAs deregulated in patients affected by ischemic heart failure.
Lnc RNAs were profiled and validated in left ventricle biopsies of 18 patients affected by non end-stage dilated ischemic cardiomyopathy and 17 matched controls.
Our technical experts have compiled a wide variety of information on our products, systems, and applications.
Whenever you need it, this database serves as a technical resource developed specifically to answer your questions and assist you with troubleshooting. It is important that you depress the pipette plunger to the second stop and create an air gap behind the sample when loading your SPRINT cartridge. These air gaps do not need to be the same size, as the instrument will correct most variation.
However, types and roles of linc RNAs in bovine mammary glands are still poorly understood.
Using computational methods, 886 unknown intergenic transcripts (UITs) were identified from five RNA-seq datasets from bovine mammary glands.
In particular, 36 linc RNAs were found in 172 milk related QTLs, whereas one linc RNA was within clinical mastitis QTL region.While it is clear that transcriptional analyses in single cells can greatly contribute to our understanding of individual and community behaviors, there continues to be a shortage of facile methods for making these measurements.This is especially the case where high-throughput measurements are required for the simultaneous analysis of thousands of cells to better assess reproducibility and variability for strong statistical analyses (21).Long noncoding RNAs (lnc RNAs) are non-protein coding transcripts regulating a variety of physiological and pathological functions.However, their implication in heart failure is still largely unknown.